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Inhibition of cell proliferation by lithium is associated with interference in cdc2 activation
Author(s) -
Smits Veronique A.J,
Essers Marieke A.G,
Loomans Dominique S.E,
Klompmaker Rob,
Rijksen Gert,
Medema René H
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01002-9
Subject(s) - lithium (medication) , cyclin dependent kinase 1 , cell cycle , cell growth , microbiology and biotechnology , mitosis , dna synthesis , p19 cell , biology , chemistry , cellular differentiation , biochemistry , endocrinology , cell , dna , adult stem cell , gene
Lithium can interfere with embryonal development in a variety of organisms. We investigated the effect of lithium on the proliferation of early embryonal cells. [ 3 H]Thymidine incorporation of non‐committed mouse P19 embryonal carcinoma cells was inhibited by lithium treatment. Similar effects were seen in a variety of other cells. This growth inhibition occurred in the G 2 phase, since cells accumulated with a 4N DNA content, but the appearance of mitotic cells was blocked. Lithium could also prevent the activation of cdc2, thereby inhibiting cyclin B/cdc2 kinase activity. These data indicate that lithium might disturb embryonal development through interference in embryonal cell cycle regulation.