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Cloning, tissue distribution, subcellular localization and overexpression of murine histidine‐rich Ca 2+ binding protein
Author(s) -
Ridgeway Alan G.,
Petropoulos Helen,
Siu Aaron,
Ball Judy K.,
Skerjanc Ilona S.
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00993-x
Subject(s) - cloning (programming) , histidine , subcellular localization , chemistry , microbiology and biotechnology , tissue distribution , biochemistry , biology , gene , enzyme , physiology , computer science , programming language
The histidine‐rich Ca 2+ binding protein (HRC) resides in the sarcoplasmic reticulum of muscle and binds Ca 2+ . Since Ca 2+ concentrations can regulate gene expression via calcineurin, the mouse homologue of HRC (mHRC) was isolated and characterized. mHRC was detected in muscle progenitor cells, in primary clonal thymic tumors and a tumor cell line, suggesting a broader role for mHRC than in Ca 2+ storage during muscle contraction. mHRC was present in the perinuclear region of myoblasts. To examine if it can regulate gene expression, mHRC was overexpressed in cells differentiating into cardiac and skeletal muscle. mHRC had no effect on cardiogenesis or myogenesis. Therefore, if mHRC plays a role in the regulation of gene expression during cellular differentiation, it does not appear to be either rate‐limiting or inhibitory.