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Insertional gene fusion technology
Author(s) -
Doi Nobuhide,
Yanagawa Hiroshi
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00991-6
Subject(s) - fusion gene , fusion protein , gene , computational biology , insertional mutagenesis , biology , fusion , genetic enhancement , gene expression , lipid bilayer fusion , recombinant dna , genetics , genome , membrane , linguistics , philosophy
The classical ‘end to end’ gene fusion technique has widely been used for monitoring gene expression, biological screening and purification of recombinant proteins. Recent progress with the ‘insertional’ gene fusion approach, on the other hand, has demonstrated that this technique can be utilized for membrane protein topology analysis, display of randomized protein libraries and design of biosensor proteins. In this review, we describe examples of insertional gene fusion and compare the old and new gene fusion techniques.