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Induction of uncoupling protein (UCP) 2 in primary cultured hepatocytes
Author(s) -
Kimura Kazuhiro,
Jung Bae Dong,
Kanehira Katsushi,
Irie Yukiko,
Cañas Xavier,
Saito Masayuki
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00987-4
Subject(s) - mitochondrion , hepatocyte , uncoupling protein , oxygen , biochemistry , biology , messenger rna , atp synthase , chemistry , microbiology and biotechnology , in vitro , gene , organic chemistry , adipose tissue , brown adipose tissue
Uncoupling protein 2 (UCP2) mRNA expression and function was examined in rat primary cultured hepatocytes. UCP2 mRNA was not expressed in freshly isolated hepatocytes, but appeared during a 24–144 h primary culture period. Isolated mitochondria from 144 h cultured hepatocytes showed a lower oxygen consumption rate in the presence of succinate and ADP. However, the ratio of the oxygen consumption rate when media contained succinate alone to that with succinate and ADP was increased by 166% versus control mitochondria. Moreover, the mitochondrial potential in the presence of succinate was decreased by 60%, indicating the potential role of UCP2 in hepatocyte mitochondria as an active uncoupler.