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Caspases‐3 and ‐7 are activated in goniothalamin‐induced apoptosis in human Jurkat T‐cells
Author(s) -
Inayat-Hussain Salmaan H.,
Osman Annuar B.,
Din Laily B.,
Ali Abdul M.,
Snowden Roger T.,
MacFarlane Marion,
Cain Kelvin
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00984-9
Subject(s) - jurkat cells , apoptosis , caspase , chemistry , microbiology and biotechnology , intrinsic apoptosis , cancer research , biology , immunology , programmed cell death , biochemistry , t cell , immune system
Goniothalamin, a plant styrylpyrone derivative isolated from Goniothalamus andersonii , induced apoptosis in Jurkat T‐cells as assessed by the externalisation of phosphatidylserine. Immunoblotting showed processing of caspases‐3 and ‐7 with the appearance of their catalytically active large subunits of 17 and 19 kDa, respectively. Activation of these caspases was further evidenced by detection of poly(ADP‐ribose) polymerase cleavage (PARP). Pre‐treatment with the caspase inhibitor benzyloxycarbonyl‐Val‐Ala‐Asp fluoromethyl ketone (Z‐VAD.FMK) blocked apoptosis and the resultant cleavage of these caspases and PARP. Our results demonstrate that activation of at least two effector caspases is a key feature of goniothalamin‐induced apoptosis in Jurkat T‐cells.