Premium
Molecular cloning of haploid germ cell‐specific tektin cDNA and analysis of the protein in mouse testis
Author(s) -
Iguchi Naoko,
Tanaka Hiromitsu,
Fujii Takayuki,
Tamura Kazutoshi,
Kaneko Yoshioki,
Nojima Hiroshi,
Nishimune Yoshitake
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00967-9
Subject(s) - biology , complementary dna , cdna library , microbiology and biotechnology , blot , microtubule , cloning (programming) , messenger rna , molecular cloning , germ cell , gene , genetics , computer science , programming language
Tektins are a class of proteins that form filamentous polymers in the walls of ciliary and flagellar microtubules. We report here the molecular cloning of a new member of the tektin family, tektin‐t, identified from a mouse haploid germ cell‐specific cDNA library. Tektin‐t mRNA encodes a protein of 430 deduced amino acids possessing RSNVELCRD, the conserved sequence of tektin family proteins. Western blotting showed a single band having a molecular weight of 86 kDa in the mouse testis. Immunohistochemistry of the testis showed that tektin‐t is localized in the flagella of elongating spermatids from developmental step 15 to maturity.