Premium
The autocrine motility factor receptor gene encodes a novel type of seven transmembrane protein 1
Author(s) -
Shimizu Kimihiro,
Tani Masachika,
Watanabe Hideomi,
Nagamachi Yasuhiro,
Niinaka Yasufumi,
Shiroishi Toshihiko,
Ohwada Susumu,
Raz Avraham,
Yokota Jun
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00966-7
Subject(s) - leucine zipper , biology , transmembrane domain , transmembrane protein , gene , peptide sequence , amino acid , microbiology and biotechnology , genetics , receptor
Autocrine motility factor receptor (AMFR) is a cell surface glycoprotein of molecular weight 78 000 (gp78), mediating cell motility signaling in vitro and metastasis in vivo. Here, we cloned the full‐length cDNAs for both human and mouse AMFR genes. Both genes encode a protein of 643 amino acids containing a seven transmembrane domain, a RING‐H2 motif and a leucine zipper motif and showed a 94.7% amino acid sequence identity to each other. Analysis of the amino acid sequence of AMFR with protein databases revealed no significant homology with all known seven transmembrane proteins, but a significant structural similarity to a hypothetical protein of Caenorhabditis elegans , F26E4.11. Thus, AMFR is a highly conserved gene which encodes a novel type of seven transmembrane protein.