Premium
Comparison of the effects of bax ‐expression in yeast under fermentative and respiratory conditions: investigation of the role of adenine nucleotides carrier and cytochrome c
Author(s) -
Priault Muriel,
Camougrand Nadine,
Chaudhuri Bhabatosh,
Schaeffer Jacques,
Ma Stéphen
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00957-6
Subject(s) - yeast , cytochrome c , biology , nucleotide , cytochrome , biochemistry , programmed cell death , saccharomyces cerevisiae , gene , microbiology and biotechnology , apoptosis , enzyme
A new system for bax ‐expression in yeast has been devised to investigate bax 's effect under fermentative and respiro‐fermentative conditions. This has allowed us to show unambiguously that the ability of bax to kill yeast is higher under respiratory conditions than under purely fermentative conditions. The extent of killing under respiro‐fermentative conditions (non‐repressive sugars) is intermediate. It has been proposed that the two proteins adenine nucleotides carrier (ANC) and cytochrome c play a crucial role in bax ‐induced cell death. We have investigated the effects of deletion of the genes encoding the two proteins on the toxicity induced by bax , using this new system. The absence of ANC did not modify bax ‐induced lethality in any way. Moreover, the absence of cytochrome c also did not prevent bax ‐induced death. Only the kinetics of lethality were altered. All these effects are prevented by co‐expression of bcl‐x L .