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Identification of a novel gene of the X,K‐ATPase β‐subunit family that is predominantly expressed in skeletal and heart muscles 1
Author(s) -
Pestov Nikolay B.,
Adams Gail,
Shakhparonov Mikhail I.,
Modyanov Nikolai N.
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00954-0
Subject(s) - skeletal muscle , isozyme , protein subunit , gene , homology (biology) , p type atpase , biology , microbiology and biotechnology , atpase , beta (programming language) , gene family , peptide sequence , amino acid , biochemistry , enzyme , gene expression , endocrinology , computer science , programming language
We have identified the fifth member of the mammalian X,K‐ATPase β‐subunit gene family. The human and rat genes are largely expressed in skeletal muscle and at a lower level in heart. The deduced human and rat proteins designated as β muscle (β m ) consist of 357 and 356 amino acid residues, respectively, and exhibit 89% identity. The sequence homology of β m proteins with known Na,K‐ and H,K‐ATPase β‐subunits are 30.5–39.4%. Unlike other β‐subunits, putative β m proteins have large N‐terminal cytoplasmic domains containing long Glu‐rich sequences. The data obtained indicate the existence of hitherto unknown X,K‐ATPase (most probably Na,K‐ATPase) isozymes in muscle cells.