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Organisation of mitochondria in living sensory neurons
Author(s) -
Dedov Vadim N,
Roufogalis Basil D
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00951-5
Subject(s) - mitochondrion , microbiology and biotechnology , neurite , confocal microscopy , biology , intracellular , biophysics , chemistry , biochemistry , in vitro
In this work, we have examined the mitochondrial organisation in living cultured primary dorsal root ganglion (DRG) neurons. Confocal microscopy and the mitochondrial potential‐sensitive fluorescent dye 5,5′,6,6′‐tetrachloro‐1,1′,3,3′‐tetraethylbenzimidazolo carbocyanine iodide (JC‐1) were used to visualise intracellular structures with a high and low membrane potential. Three‐dimensional reconstruction revealed a mitochondrial organisation featuring separate highly polarised mitochondria, clusters of mitochondria located mainly at the base of neurite hillocks and filamentous mitochondrial structures. Filamentous mitochondria were distributed along the cell body, especially between neurites. A functional integration between mitochondrial structures is proposed.