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Co‐introduction of an antisense gene for an endogenous seed storage protein can increase expression of a transgene in Arabidopsis thaliana seeds
Author(s) -
Goossens Alain,
Van Montagu Marc,
Ange Geert
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00943-6
Subject(s) - transgene , arabidopsis thaliana , biology , gene , storage protein , endogeny , gene expression , arabidopsis , genetically modified crops , phaseolus , microbiology and biotechnology , genetics , biochemistry , botany , mutant
We have investigated whether the expression in Arabidopsis thaliana seeds of a transgene (the Phaseolus vulgaris arcelin (arc)5‐I gene) could be enhanced by the simultaneous introduction of an antisense gene for an endogenous seed storage protein (2S albumin). Seeds of plants transformed with both the arc5‐I gene and a 2S albumin antisense gene contained reduced amounts of 2S albumins and increased arcelin‐5 (Arc5) accumulation levels compared to lines harboring the arc5‐I gene only. Arc5 production could be enhanced to more than 24% of the total seed protein content, suggesting that antisense technology could be of great utility to favor high expression of transgenes.