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Involvement of Sp1 in basal and retinoic acid induced transcription of the human tissue‐type plasminogen activator gene
Author(s) -
Merchiers Pascal,
Bulens Frank,
De Vriese Astrid,
Collen Désiré,
Belayew Alexandra
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00942-4
Subject(s) - enhancer , retinoic acid , microbiology and biotechnology , activator (genetics) , plasminogen activator , response element , transcription (linguistics) , biology , reporter gene , promoter , chemistry , gene , transcription factor , gene expression , biochemistry , genetics , linguistics , philosophy
Transcription of the human tissue‐type plasminogen activator (t‐PA) gene is regulated by a multi‐hormonal responsive enhancer at −7 kb. Transient co‐transfections of Drosophila SL2 and human HT1080 fibrosarcoma cells with t‐PA reporter constructs showed that Sp1 and Sp3 activate the t‐PA promoter. Moreover Sp1 (but not Sp3) binding to the promoter is involved in induction by retinoic acid (RA), a response mediated through the enhancer. The role of Sp1 is specific, since mutation of the CRE element in the promoter did not affect response to RA. In contrast, the glucocorticoid induction mediated by the enhancer is independent of these Sp1 and CRE elements.