Premium
Generation of multiple transcripts from the chicken chondromodulin‐I gene and their expression during embryonic development
Author(s) -
Shukunami Chisa,
Yamamoto Saori,
Tanabe Tadashi,
Hiraki Yuji
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00931-x
Subject(s) - biology , in situ hybridization , embryo , morphogenesis , embryonic stem cell , microbiology and biotechnology , embryogenesis , gene , cartilage , polyadenylation , gene expression , messenger rna , complementary dna , anatomy , genetics
Chondromodulin‐I (ChM‐I) is an angiogenesis inhibitor isolated from fetal bovine cartilage. Here, we report the nucleotide sequence of chicken ChM‐I cDNA. Chicken mature ChM‐I had a significantly larger N‐terminal hydrophilic domain than its mammalian counterparts. Chicken embryos expressed multiple transcripts (3.3, 2.0 and 1.7 kb in size) due to the alternative utilization of polyadenylation signals, whereas only the 1.7 kb transcripts were detected in mammals. Although confined to cartilage and eye at a later stage of development, whole‐mount in situ hybridization revealed the expression of ChM‐I mRNA in somites, heart, bronchial arches, roof plate, retina and limb buds. The expression pattern of the gene suggests a role for ChM‐I in the morphogenesis during embryonic development.