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In vitro production of cytokines is influenced by sulfatide and its precursor galactosylceramide
Author(s) -
Bovin Lone Frier,
Fredman Pam,
Månsson Jan-Eric,
Buschard Karsten,
Bendtzen Klaus
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00908-4
Subject(s) - secretion , lipopolysaccharide , cytokine , tumor necrosis factor alpha , proinflammatory cytokine , chemistry , interleukin , in vitro , peripheral blood mononuclear cell , interleukin 6 , endocrinology , medicine , biology , immunology , inflammation , biochemistry
Effects of sulfatide and its precursor galactosylceramide (gal‐cer) on the kinetics of production of cytokines were studied. In human mononuclear leucocytes, gal‐cer but not sulfatide induced significantly increased amounts of interleukin (IL)‐1β, IL‐6 and tumor necrosis factor (TNF) mRNA. In phytohemagglutinin‐stimulated cultures, gal‐cer increased the levels of IL‐1β and IL‐6 mRNA and secreted IL‐1β and IL‐6, while sulfatide decreased the amounts of IL‐6 mRNA and secreted IL‐6. Gal‐cer also increased TNF secretion. In lipopolysaccharide‐stimulated cells, sulfatide but not gal‐cer decreased the secretion of IL‐1β and IL‐10, a potent suppressor of production of many cytokines. Thus, sulfatide and gal‐cer affect cytokine production differently, most likely at the level of gene expression. This may have implications in diseases where inflammatory cytokines play a pathogenic role.