Premium
Murine leukemia provirus‐mediated activation of the Notch1 gene leads to induction of HES‐1 in a mouse T lymphoma cell line, DL‐3
Author(s) -
Lee Jong-Seo,
Ishimoto Akinori,
Honjo Tasuku,
Yanagawa Shin-ichi
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00901-1
Subject(s) - provirus , biology , leukemia , lymphoma , cell culture , microbiology and biotechnology , gene , murine leukemia virus , carcinogenesis , fusion gene , cancer research , fusion protein , enhancer , t cell leukemia , jurkat cells , t cell , gene expression , genetics , immunology , recombinant dna , genome , immune system
Constitutive activation of Notch signaling is known to be associated with tumorigenesis. In a mouse T lymphoma cell line, DL‐3, we found that a murine leukemia provirus was inserted in the Notch1 locus, which led to marked expression of a virus‐Notch1 fusion mRNA encoding an intracellular portion of the Notch1 protein. Furthermore, expression and nuclear localization of this constitutively active form of Notch1 protein were confirmed. Corresponding to this finding, the transcription of the hairy/enhancer of split ( HES‐1 ) gene, a known target of Notch1 signaling, was elevated in this cell line. A potential role for overexpressed HES‐1 in the development of the lymphoma was discussed.