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Translocon‐associated protein α transcripts are induced by granulocyte‐macrophage colony‐stimulating factor and exhibit complex alternative polyadenylation
Author(s) -
Hirama Toshiyasu,
Miller Carl W.,
Koeffler H.Phillip
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00885-6
Subject(s) - polyadenylation , biology , gene , complementary dna , microbiology and biotechnology , cloning (programming) , translocon , genetics , messenger rna , chromosomal translocation , computer science , programming language
The cloning of full length cDNA for the translocon‐associated protein α subunit, previously called signal sequence receptor α, is reported as a result of differential display experiments in search of genes induced by granulocyte‐macrophage colony‐stimulating factor. Its messenger RNA was more abundant in growing cells than in either factor‐deprived cells or quiescent cells and comprised four species, each having microheterogeneity, as a result of complex alternative polyadenylation apparently dependent on arrays of non‐canonical polyadenylation signals. Radiation hybrid mapping of the gene showed that the gene is on the short arm of chromosome 6.