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Activation and expression of ERK, JNK, and p38 MAP‐kinases in isolated islets of Langerhans: implications for cultured islet survival
Author(s) -
Paraskevas Steven,
Aikin Reid,
Maysinger Dusica,
Lakey Jonathan R.T.,
Cavanagh Thomas J.,
Hering Bernhard,
Wang Rennian,
Rosenberg Lawrence
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00882-0
Subject(s) - p38 mitogen activated protein kinases , islet , mapk/erk pathway , kinase , mitogen activated protein kinase , microbiology and biotechnology , regulator , isolation (microbiology) , chemistry , biology , endocrinology , biochemistry , diabetes mellitus , gene
Isolation and purification of islet cells exposes them to ischemic, osmotic and mechanical stresses. The objective of this study was to determine the roles of the MAP‐kinases in islets immediately following isolation. During the first 48 h, activity of JNK1 and JNK2 declined markedly. Activity of p38 increased steadily with time in culture while extracellular signal regulated kinase (ERK) activity declined dramatically within 24 h post‐isolation. High p38 activation relative to ERK activation immediately following isolation correlated with a decrease in islet survival after 36 h in culture. Absence and/or transiency of ERK signaling in conjunction with sustained activation of p38 pathway could be an important regulator of cell death in islets during and following their isolation by commonly employed procedures.