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The human secretin receptor gene: genomic organization and promoter characterization
Author(s) -
Ho Po-ki,
Fong Rango S.M.,
Kai Heidi S.T.,
Lau Elisa H.Y.,
Ngan Elly S.W.,
Cotton Calvin U.,
Chow Billy K.C.
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00864-9
Subject(s) - secretin , gene , reporter gene , microbiology and biotechnology , promoter , biology , exon , intron , alternative splicing , gene expression , genetics , secretion , biochemistry
Secretin is the most potent regulator of pancreatic bicarbonate, electrolyte and volume secretion. In this report, the organization of the human secretin receptor (hSR) gene was characterized by overlapping genomic phage clones. The hSR gene consists of 13 exons and 12 introns with all the splice donor and acceptor sites conforming to the canonical GT/AG rule. By transient reporter gene assays, the wild‐type promoter, containing 3.0 kb of the hSR gene 5′ flanking region, was able to drive 5.8±0.6 and 6.6±0.2‐fold ( P <0.01) increases in luciferase activities in pancreatic ductule‐derived PANC‐1 and BPD‐1 cells, respectively. By subsequent 5′ and 3′ deletion analysis, a promoter element was identified within −408 to −158, relative to the ATG codon. This promoter element was found to be cell‐specific since it could drive reporter gene expression in PANC‐1 and BPD‐1 cells but not in Hs 262.St, Hs 746T and αT3‐1 cells. The study of the transcriptional control of human secretin and its receptor should shed light on the pathological developments of pancreatic cancer and autism in the future.