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Existence of uncoupling protein‐2 antigen in isolated mitochondria from various tissues
Author(s) -
Ježek Petr,
Žáčková Markéta,
Řeháková Zuzana,
Růžička Michal,
Borecký Jiří,
Škobisová Eva,
Brucknerová Jana,
Garlid Keith D.,
Gimeno Ruth E.,
Tartaglia Louis A.
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00853-4
Subject(s) - ucp3 , brown adipose tissue , uncoupling protein , white adipose tissue , skeletal muscle , adipose tissue , biology , hamster , mitochondrion , thermogenin , antiserum , antibody , microbiology and biotechnology , antigen , recombinant dna , endocrinology , medicine , biochemistry , immunology , gene
Antibodies against Escherichia coli ‐expressed uncoupling protein‐2 (UCP2) and uncoupling protein‐3 (UCP3) were raised by operating the blotted proteins into the spleen of minipigs. The antisera reacted more intensively with the recombinant UCP2 and UCP3 than with uncoupling protein‐1 (UCP1) isolated from brown adipose tissue. Moreover, anti ‐UCP2 and cross‐reacting anti ‐UCP3 antibodies identified the presence of the UCP2/3 antigen in isolated mitochondria from rat heart, rat kidney, rat brain, rabbit epididymal white adipose tissue, hamster brown adipose tissue, and rabbit skeletal muscle. It has been concluded that UCP2 is expressed in these tissues (UCP3 in skeletal muscle); however their existence in mitochondria had not previously been demonstrated.