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Premium Evidence for a small anion‐selective channel in the cell wall ofMycobacterium bovis BCG besides a wide cation‐selective pore
Author(s)
T. Lichtinger,
Beate Heym,
Elke Maier,
Eichner H,
Stewart T. Cole,
Roland Benz
Publication year1999
Publication title
febs letters
Resource typeJournals
PublisherElsevier BV
AbstractTwo channels were observed in extracts of whole Mycobacterium bovis BCG cells using organic solvents and detergents. The channels derived from organic solvent treatment had a single-channel conductance of about 4.0 nS in 1 M KCl in lipid bilayer membranes with properties similar to those of the channels discovered previously in Mycobacterium smegmatis and Mycobacterium chelonae. The channel was in its open configuration only at low transmembrane potentials. At higher voltages it switched to closed states that were almost impermeable for ions. Lipid bilayer experiments in the presence of detergent extracts of whole cells revealed another channel with a single-channel conductance of only 780 pS in 1 M KCl. Our results indicate that the mycolic acid layer of M. bovis BCG contains two channels, one is cation-selective and its permeability properties can be finely controlled by cell wall asymmetry or potentials. The other one is anion-selective, has a rather small single-channel conductance and is voltage-insensitive. The concentration of channel-forming proteins in the cell wall seems to be small, which is in agreement with the low cell wall permeability for hydrophilic solutes
Subject(s)bacteria , biochemistry , biology , biophysics , cell wall , chemistry , combinatorics , conductance , genetics , ion , ion channel , lipid bilayer , mathematics , medicine , membrane , membrane potential , mycobacterium , mycobacterium bovis , mycobacterium smegmatis , mycobacterium tuberculosis , organic chemistry , pathology , receptor , tuberculosis
Language(s)English
SCImago Journal Rank1.593
H-Index257
eISSN1873-3468
pISSN0014-5793
DOI10.1016/s0014-5793(99)00844-3

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