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H3‐rules: identification of CDR‐H3 structures in antibodies
Author(s) -
Shirai Hiroki,
Kidera Akinori,
Nakamura Haruki
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00821-2
Subject(s) - complementarity determining region , complementarity (molecular biology) , antibody , computational biology , heavy chain , protein tertiary structure , peptide sequence , chemistry , biology , immunoglobulin light chain , genetics , biochemistry , gene
For the third complementarity determining region of the antibody heavy chain (CDR‐H3), we propose the ‘H3‐rules’, which should identify the tertiary structure from the amino acid sequence of the CDR‐H3 segment. A total of 100 CDR‐H3 segments from well‐determined crystal structures were analyzed. Distinctive relationships between the structures and the sequences were revealed from 55 segments, and the rules were examined for the other 45 segments and were verified. In some antibodies, basic residues at specific positions were revealed to be notable signals, with their ability to form salt bridges and to assume conformations inconsistent with the rules.