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In vitro and in vivo effects of glucocorticoids on gene transfer to skeletalmuscle
Author(s) -
Serge Braun,
Christophe Jenny,
Christine Thioudellet,
Frédéric Perraud,
Marie-Christine Claudepierre,
Françoise Längle-Rouault,
Dalila Ali-Hadji,
Klaus Schughart,
Andréa Pavirani
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00818-2
Subject(s) - in vivo , in vitro , gene transfer , citation , gene , transfer (computing) , computer science , microbiology and biotechnology , information retrieval , biology , genetics , world wide web , parallel computing
As a pharmacological approach to potentially improve gene transfer efficiency into skeletal muscle cells, glucocorticoids were shown here to allow efficient transfection of cultured and mouse human myoblasts, human pulmonary A549 cells, but not dog myoblasts, independently of the transfection protocol, the reporter gene and the transcription promoter employed. Transduction with adenovirus was also increased by dexamethasone. Pretreatment of cells 48 h prior to transfection was most effective and was shown to be concentration-dependent. This effect is mediated by binding to the glucocorticoid receptor, but not by glucocorticoid responsive elements present in the vectors. The acute dexamethasone effect could be due to increased plasmid entry into the cells as suggested by Southern blot, whereas the sustained increase of luciferase activity in dexamethasone-treated cultures may be related to intracellular mechanisms following cell entry. In mice in vivo, a similar increase of luciferase activity upon glucocorticoid treatment was found.