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The zeaxanthin biosynthesis enzyme ²‐carotene hydroxylase is involvedin myxoxanthophyll synthesis in Synechocystis sp. PCC 6803
Author(s) -
Delphine Lagarde,
Wim F. J. Vermaas
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00817-0
Subject(s) - zeaxanthin , enzyme , biosynthesis , citation , biochemistry , chemistry , computer science , information retrieval , carotenoid , world wide web , lutein
Beta-carotene hydroxylase is known to be involved in zeaxanthin synthesis. Disruption of the crtR gene encoding beta-carotene hydroxylase in Synechocystis sp. PCC 6803 resulted in the absence of both zeaxanthin synthesis and myxoxanthophyll accumulation in the mutant strain. A new carotenoid was detected in this strain. Its chemical structure was close to that of myxoxanthophyll, but the hydroxyl group on the beta-ring was lacking. This compound, deoxy-myxoxanthophyll, most likely is an intermediate in the myxoxanthophyll biosynthesis pathway. Therefore, beta-carotene hydroxylase is involved not only in zeaxanthin synthesis but also in myxoxanthophyll synthesis in Synechocystis. Furthermore, myxoxanthophyll in Synechocystis was found to have a higher molecular mass than what was determined in other species. This difference is likely to be due to a difference in the sugar moiety.