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Establishment of an embryonic stem (ES) cell line derived from a non‐obese diabetic (NOD) mouse: in vivo differentiation into lymphocytes and potential for germ line transmission
Author(s) -
Nagafuchi Seiho,
Katsuta Hitoshi,
Kogawa Kazuhiko,
Akashi Tomoyuki,
Kondo Shiori,
Sakai Yumiko,
Tsukiyama Tadasuke,
Kitamura Daisuke,
Niho Yoshiyuki,
Watanabe Takeshi
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00801-7
Subject(s) - nod , biology , embryonic stem cell , stem cell , microbiology and biotechnology , chimera (genetics) , alkaline phosphatase , nod mice , immunology , in vivo , genetics , gene , biochemistry , enzyme
A non‐obese diabetic (NOD) mouse‐derived embryonic stem (ES) cell line has been stably maintained in an undifferentiated state with a characteristic ES cell‐like morphology, expressing the stem cell marker alkaline phosphatase, and displaying a normal diploid karyotype. After injecting the NOD‐ES cells into blastocysts, chimeric mice were obtained. Small but significant numbers of lymphocytes expressed the NOD‐derived MHC allele. When a chimeric mouse was mated with C57BL/6 mice, an agouti mouse was obtained, having the NOD‐derived H‐2 I‐A β g7 haplotype. Thus, an NOD‐ES cell line which can differentiate into lymphocytes with potential for germ line transmission was successfully established.