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Reduction of G‐box binding factor DNA binding activity, but not G‐box binding factor abundance, causes the downregulation of RBCS2 expression during early tomato fruit development
Author(s) -
Baum Katja,
Wienand Udo,
Meier Iris
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00784-x
Subject(s) - downregulation and upregulation , microbiology and biotechnology , biology , promoter , promoter activity , gene , gene expression , binding site , genetics
The downregulation of RBCS2 promoter activity during tomato fruit development has been investigated by transient gene expression. A major drop in promoter activity occurs between 5 and 25 mm fruit diameter, corresponding to the late cell division to early cell enlargement phase. This drop is abolished by a mutation of the single G‐box element necessary for high RBCS2 promoter activity in young tomato fruit. The G‐box binding activity of fruit nuclear and total protein extracts drops concomitantly with the reduction of RBCS2 promoter activity while G‐box binding factor expression is not affected. The data indicate that the developmental signal that downregulates the RBCS2 promoter acts on the regulation of DNA binding activity of constitutively expressed G‐box binding factors.