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The effect of 17β‐estradiol‐DNA adducts on the replication of exon # 5 of the human suppressor gene p53
Author(s) -
Yu Fu-Li,
Zheng Wei-Yun,
Wang Mian-Ying,
Bender Wanda,
Cheerva Alexandra,
Miller James
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00769-3
Subject(s) - dna , exon , microbiology and biotechnology , biology , gene , dna replication , suppressor , genetics
Using a PCR technique, exon # 5 of the human tumor suppressor gene p53 was amplified and ligated into the pCRII vector and transformed into Escherichia coli INVαF’ competent cells. The cloned exon # 5 was 184 bp long. Evidence is presented to show that after dimethyldioxirane epoxidation, 17β‐estradiol was able to form 17β‐estradiol‐DNA adducts and to strongly inhibit the replication of the cloned exon # 5 producing smaller sizes of DNA fragments and introducing errors of incorporation at the 3′‐end of the terminating DNAs. The errors occurred mainly at the clusters of the complementary ‘G’ and ‘A’ bases on the template strand DNA, presumably, the major sites where the 17β‐estradiol‐DNA adducts were formed.