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Plant cell growth and differentiation may involve GAP regulation of Rac activity
Author(s) -
Borg Søren,
Pødenphant Lone,
Jensen Trine Juul,
Poulsen Carsten
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00750-4
Subject(s) - gtpase , lotus japonicus , subfamily , biology , recombinant dna , microbiology and biotechnology , eukaryote , gtpase activating protein , biochemistry , gene , signal transduction , g protein , genome , mutant
Two Rac GTPase cDNAs, LjRac1 and LjRac2 , were identified in the legume Lotus japonicus . Two‐hybrid screening with dominant‐constitutive mutations in the two Rac GTPases target three plant cDNAs, LjRacGAP1 , LjRacGAP2 and LjRacGAP3 , that encode putative GTPase activating proteins of Rho‐GTPase subfamily members. Employing Rac antiserum, purified recombinant LjRac GTPases and recombinant LjRacGAP1, for ligand overlay assays, in vitro GAP affinity assays and GTPase activation, we confirmed that eukaryote Rac/RacGAP interplay is conserved in plants. In this investigation we have developed some tools that can be used to characterize the role of enhanced LjRac2 expression in developing root nodules.