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Selective activation of phospholipase D2 by unsaturated fatty acid
Author(s) -
Kim Jae Ho,
Kim Yong,
Lee Sang Do,
Lopez Isabel,
Arnold Rebecca S.,
Lambeth J.David,
Suh Pann-Ghill,
Ryu Sung Ho
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00745-0
Subject(s) - chemistry , fatty acid , biochemistry
Although oleate has been implicated in the regulation of phospholipase D (PLD) activity, the molecular identity of the oleate‐stimulated PLD is still poorly understood. We now report that oleate selectively stimulates the enzymatic activity of PLD2 but not of PLD1, with an optimal concentration of 20 μM in vitro. Intriguingly, phosphatidylinositol 4,5‐bisphosphate (PIP 2 ) synergistically stimulates the oleate‐dependent PLD2 activity with an optimal concentration of 2.5 μM. These results provide the first evidence that oleate is a PLD2‐specific activating factor and PLD2 activity is synergistically stimulated by oleate and PIP 2 .