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N ‐Acetyl‐sphingenine‐1‐phosphate is a potent calcium mobilizing agent
Author(s) -
Gijsbers Sofie,
Mannaerts Guy P.,
Himpens Bernard,
Van Veldhoven Paul P.
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00735-8
Subject(s) - thapsigargin , ryanodine receptor , chemistry , calcium , intracellular , calcium in biology , phosphate , biophysics , biochemistry , endocrinology , biology , organic chemistry
Calcium mobilization induced by phosphorylated sphingoid bases was analyzed in calf pulmonary artery endothelial cells by confocal microscopy. A sphingenine‐1‐phosphate (SeP) analogue, N ‐acetyl‐sphingenine‐1‐phosphate (N‐C 2 ‐SeP), exogenously added to these cells, caused a fast and transient intracellular rise in calcium and was as potent as SeP. A minimal concentration of 0.6 nM for N‐C 2 ‐SeP versus 1 nM for SeP was determined. The N‐C 2 ‐SeP‐induced Ca 2+ ‐signaling, like the response to SeP, was due to a release from thapsigargin‐sensitive, ryanodine‐insensitive, intracellular Ca 2+ ‐stores and not to a Ca 2+ ‐influx. N‐C 2 ‐SeP can be considered as a truncated ceramide‐phosphate, a lipid already reported to be mitogenic (Gomez‐Munoz, A., Duffy, P.A., Martin, A., O'Brien, L., Byun, H.S., Bittman, R. and Brindley, D.N. (1995) Mol. Pharmacol. 47, 833–839), an effect that might be secondary to Ca 2+ ‐mobilization.