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Analysis of the in vivo interaction between a basic repressor and an acidic activator
Author(s) -
Wellhausen Anne,
Lehming Norbert
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00718-8
Subject(s) - repressor , psychological repression , activator (genetics) , in vivo , transcription (linguistics) , chemistry , transcription factor , microbiology and biotechnology , in vitro , biophysics , biochemistry , biology , gene , gene expression , genetics , linguistics , philosophy
The artificial basic repressor SSB24 represses transcription of a reporter construct activated by GCN4. We show that the positively charged SSB24 and the negatively charged acidic activator GCN4 interact in vitro and in vivo. However, deleting the interaction domain from the GCN4 activator does not result in loss of repression by SSB24. Similarly, transcription activated by the holoenzyme component SRB2 is repressed, although SSB24 and SRB2 do not interact. Repression by SSB24 therefore does not depend on the observed protein‐protein interaction between SSB24 and GCN4.