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Cloning of a novel kinase (SIK) of the SNF1/AMPK family from high salt diet‐treated rat adrenal 1
Author(s) -
Wang Zhi-g,
Takemori Hiroshi,
Halder Sunil K,
aka Yasuki,
Okamoto Mitsuhiro
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00708-5
Subject(s) - complementary dna , northern blot , threonine , protein kinase a , ampk , autophosphorylation , kinase , messenger rna , zona fasciculata , biology , serine , amino acid , microbiology and biotechnology , clone (java method) , gene , endocrinology , medicine , adrenal cortex , biochemistry , phosphorylation
PCR‐coupled cDNA subtraction hybridization was adapted to identify the genes expressed in the adrenocortical tissues from high salt diet‐treated rat. A novel cDNA clone, termed alt‐ nducible inase (SIK), encoding a polypeptide (776 amino acids) with significant similarity to protein serine/threonine kinases in the SNF1/AMPK family was isolated. An in vitro kinase assay demonstrated that SIK protein had autophosphorylation activity. Northern blot revealed that SIK mRNA levels were markedly augmented by ACTH treatment both in rat adrenal glands and in Y1 cells. SIK may play an important role in the regulation of adrenocortical functions in response to high plasma salt and ACTH stimulation.