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Hypochlorite modification of high density lipoprotein: effects on cholesterol efflux from J774 macrophages
Author(s) -
Bergt Constanze,
Reicher Helga,
Malle Ernst,
Sattler Wolfgang
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00677-8
Subject(s) - efflux , hypochlorite , cholesterol , chemistry , lipoprotein , environmental chemistry , biochemistry , inorganic chemistry
The present study was aimed at investigating effects of hypochlorite (HOCl) modification of high density lipoproteins subclass 3 (HDL 3 ) on their ability for cellular cholesterol removal from permanent J774 macrophages. Our findings indicate that HOCl (added as reagent or generated enzymatically by the myeloperoxidase/H 2 O 2 /Cl − system) damages apolipoprotein A‐I, the major protein component of HDL 3 . Fatty acid analysis of native and HOCl‐modified HDL 3 revealed that unsaturated fatty acids in both major lipid subclasses (phospholipids and cholesteryl esters) are targets for HOCl attack. HOCl modification resulted in impaired HDL 3 ‐mediated cholesterol efflux from J774 cells, regardless of whether reagent or enzymatically generated HOCl was used to modify the lipoprotein. Decreased cholesterol efflux was also observed after HOCl modification of reconstituted HDL particles. Impairment of cholesterol efflux from macrophages was noticed at low and physiologically occurring HOCl concentrations.