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A RNA polymerase with transcriptional activity at 0°C from the Antarctic bacterium Pseudomonas syringae
Author(s) -
Uma S.,
Jadhav R.S.,
Seshu Kumar G.,
Shivaji S.,
Ray M.K.
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00660-2
Subject(s) - rna polymerase , rna dependent rna polymerase , polymerase , pseudomonas syringae , microbiology and biotechnology , transcription (linguistics) , biology , rna polymerase i , sigma factor , rna polymerase ii , specificity factor , rna , escherichia coli , enzyme , biochemistry , gene , gene expression , promoter , linguistics , philosophy
A DNA‐dependent RNA polymerase was purified from the Antarctic psychrotrophic bacterium Pseudomonas syringae . The RNA polymerase showed a typical eubacterial subunit composition with β, β′, α 2 and σ subunits. The subunits cross‐reacted with antibodies raised against holoenzyme and the individual subunits of the RNA polymerase of Escherichia coli . However, the enzyme was considered unique, since unlike the RNA polymerase of mesophilic E. coli it exhibited significant and consistent transcriptional activity (10–15%) even at 0°C. But, similar to the enzyme from the mesophilic bacterium, the RNA polymerase from P. syringae exhibited optimum activity at 37°C. The study also demonstrates that the RNA polymerase of P. syringae could preferentially transcribe the cold‐inducible gene cspA of E. coli only at lower temperatures (0–22°C). The polymerase was also observed to be relatively more rifampicin‐resistant during transcription at lower temperature.