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Genomic organization of three neurotoxins active on small conductance Ca 2+ ‐activated potassium channels from the scorpion Buthus martensi Karsch 1
Author(s) -
Wu Jing-Jiang,
Dai Li,
Lan Zheng-Dao,
Chi Cheng-Wu
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00651-1
Subject(s) - complementary dna , microbiology and biotechnology , signal peptide , peptide , toxin , genomic dna , chemistry , venom , neurotoxin , biology , peptide sequence , gene , biochemistry
According to the known primary sequences of three neurotoxins active on small conductance Ca 2+ ‐activated potassium channels from the scorpion Buthus martensi Karsch, their corresponding cDNAs were cloned and sequenced using 3′‐ and 5′‐RACE. All of them encoded a signal peptide composed of 28 residues and a mature toxin of 29, 28 and 33 residues, respectively. Their cDNA deduced sequences were totally consistent with those determined, and the C‐terminal amidation of one neurotoxin was confirmed. The genomic DNAs of these three toxins were also amplified by PCR, cloned and sequenced. They all consisted of two exons disrupted by a small single intron. All of these introns were inserted within the signal peptide at the same −10 position upstream from the mature toxin, consisting of 94, 78 and 87 bp, respectively.