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In vivo synthesis of complex N ‐glycans by expression of human N ‐acetylglucosaminyltransferase I in the filamentous fungus Trichoderma reesei
Author(s) -
Maras Marleen,
De Bruyn André,
Vervecken Wouter,
Uusitalo Jaana,
Penttilä Merja,
Busson Roger,
Herdewijn Piet,
Contreras Roland
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00648-1
Subject(s) - trichoderma reesei , in vivo , filamentous fungus , chemistry , strain (injury) , fungus , gene , glycan , biology , biochemistry , cellulase , enzyme , botany , genetics , anatomy , glycoprotein
The human N ‐acetylglucosaminyltransferase I gene was introduced in the genome of Trichoderma reesei strain VTT‐D‐80133. Expression was studied after induction from the cellobiohydrolase I promoter. Successful in vivo transfer of GlcNAc was demonstrated by analyzing the neutral N ‐glycans which were synthesized on cellobiohydrolase I. Final proof of the formation of GlcNAcMan 5 GlcNAc 2 was obtained by NMR analysis.