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New substrates of DNA polymerases
Author(s) -
Victorova Lyubov,
Sosunov Vasily,
Skoblov Alexander,
Shipytsin Alexander,
Krayevsky Alexander
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00615-8
Subject(s) - reverse transcriptase , dna polymerase , dna , nucleotide , polymerase , substrate (aquarium) , enzyme , chemistry , biochemistry , hydrolysis , substrate specificity , stereochemistry , biology , polymerase chain reaction , ecology , gene
Bis‐(2′‐deoxynucleoside) 5′,5′‐tetraphosphates and bis‐(2′‐deoxynucleoside) 5′,5′‐triphosphates were shown to be a new type of substrate for several DNA polymerases of human, bacterial and viral origin. Their substrate properties depend both on their structure and on the nature of the enzyme. They are incorporated by both termini in correspondence with the template nucleotide program in the active center. The results obtained support the mechanism of their direct incorporation rather than prior hydrolysis to dNTP. The highest activity of these compounds was observed for HIV reverse transcriptase. The probable biological significance of the reaction is discussed.