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Targeting of tail‐anchored proteins to yeast mitochondria in vivo
Author(s) -
Egan Billie,
Beilharz Traude,
George Rebecca,
Isenmann Sandra,
Gratzer Sabine,
Wattenberg Binks,
Lithgow Trevor
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00581-5
Subject(s) - transmembrane protein , protein targeting , translocase of the inner membrane , microbiology and biotechnology , mitochondrial membrane transport protein , translocase , transmembrane domain , translocase of the outer membrane , mitochondrion , membrane protein , mitochondrial carrier , biology , translation (biology) , membrane , inner mitochondrial membrane , chemistry , biochemistry , bacterial outer membrane , messenger rna , gene , receptor , chromosomal translocation , escherichia coli
Tail‐anchored proteins are inserted into intracellular membranes via a C‐terminal transmembrane domain. The topology of the protein is such that insertion must occur post‐translationally, since the insertion sequence is not available for membrane insertion until after translation of the tail‐anchored polypeptide is completed. Here, we show that the targeting information in one such tail‐anchored protein, translocase in the outer mitochondrial membrane 22, is contained in a short region flanking the transmembrane domain. An equivalent region is sufficient to specify the localisation of Bcl2 and SNARE proteins to the secretory membranes. We discuss the targeting process for directing members of this protein family to the secretory and mitochondrial membranes in vivo.