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Sequence of subunit a of the Na + ‐translocating F 1 F 0 ‐ATPase of Acetobacterium woodii : proposal for residues involved in Na + binding
Author(s) -
Rahlfs Stefan,
Müller Volker
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00576-1
Subject(s) - protein subunit , transmembrane domain , atpase , chemistry , stereochemistry , enzyme , crystallography , microbiology and biotechnology , biology , biochemistry , gene
Na + transport through the F 0 domain of Na + ‐F 1 F 0 ‐ATPases involves the combined action of subunits c and a but the residues involved in Na + liganding in subunit a are unknown. As a first step towards the identification of these residues, we have cloned and sequenced the gene encoding subunit a of the Na + ‐F 1 F 0 ‐ATPase of Acetobacterium woodii . This is the second sequence available now for this subunit from Na + ‐F 1 F 0 ‐ATPases. A comparison of subunit a from Na + ‐F 1 F 0 ‐ATPases with those from H + ‐translocating enzymes unraveled structural similarity in a C‐terminal segment including the ultimate and penultimate transmembrane helix. Seven residues are conserved in this region and, therefore, likely to be involved in Na + liganding.