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Major differences in oxysterol formation in human low density lipoproteins (LDLs) oxidized by OH/O 2 − free radicals or by copper
Author(s) -
Zarev Stéphanie,
Thérond Patrice,
Bonnefont-Rousselot Dominique,
Beaudeux Jean-Louis,
Gardès-Albert Monique,
Legrand Alain
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00564-5
Subject(s) - oxysterol , radical , copper , chemistry , cholesterol , biochemistry , organic chemistry
The aim of our study was to determine the oxysterol formation in low density lipoproteins (LDLs) oxidized by defined oxygen free radicals (OH/O 2 − ). This was compared to the oxysterol produced upon the classical copper oxidation procedure. The results showed a markedly lower formation of oxysterols induced by OH/O 2 − free radicals than by copper and thus suggested a poor ability of these radicals to initiate cholesterol oxidation in LDLs. Moreover, the molecular species of cholesteryl ester hydroperoxides produced by LDL copper oxidation seemed more labile than those formed upon OH/O 2 − ‐induced oxidation, probably due to their degradation by reaction with copper ions.