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Characterization of an alternatively spliced isoform of rat vesicle associated membrane protein‐2 (VAMP‐2)
Author(s) -
Mandic Robert,
Lowe Anson W.
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00551-7
Subject(s) - rna splicing , alternative splicing , gene isoform , microbiology and biotechnology , cell fractionation , messenger rna , intron , colocalization , chemistry , rna , biochemistry , biology , gene , membrane
VAMPs are esicle ssociated embrane roteins that are essential for secretion. A spliced isoform of rat VAMP‐2, called VAMP‐2B, is characterized in this study. The VAMP‐2B transcript is the result of alternative RNA splicing in which an intron is retained. The predicted amino acid sequence of VAMP‐2B differs from VAMP‐2 at its carboxy‐terminal end. Because recent studies have shown that VAMP's carboxy‐terminal end influences the protein's sorting, the location of myc‐epitope tagged VAMP‐2B in PC12 cells was determined. Subcellular fractionation showed colocalization of myc‐VAMP‐2B and endogenous VAMP‐2. Thus alternative RNA splicing does not affect VAMP‐2 sorting in PC12 cells.