Premium
LIS1 and platelet‐activating factor acetylhydrolase (Ib) catalytic subunits, expression in the mouse oocyte and zygote
Author(s) -
Cahana Aviv,
Reiner Orly
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00548-7
Subject(s) - zygote , biology , oocyte , microbiology and biotechnology , embryo , protein subunit , gene isoform , chemistry , embryogenesis , gene , genetics
Platelet‐activating factor is a phospholipid with several documented roles in the pre‐implantation embryo. Enzymes that belong to the platelet‐activating factor acetylhydrolases family inactivate platelet‐activating factor. Cytosolic platelet‐activating factor acetylhydrolase (Ib) is a heterotetramer composed of two catalytic subunits (α1/α2) and two regulatory LIS1 subunits. The expression of these components was monitored in the mouse oocytes and zygotes using reverse‐transcribed PCR and Western blot analysis. Interestingly, these proteins are expressed in the oocyte and zygote and their expression increases after fertilization, probably due to stabilization of maternal RNA. Lis1 mRNA transcription also increases after fertilization. However, assaying for expression of a specific paternal LIS1 isoform detected no zygotic translation in the one cell stage. These findings suggest a potential role for platelet‐activating factor acetylhydrolase (Ib) components in the early mouse embryo.