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Characterization of a novel cis ‐benzene dihydrodiol dehydrogenase from Pseudomonas putida ML2
Author(s) -
Fong Karen P.Y,
Tan Hai-Meng
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00520-7
Subject(s) - pseudomonas putida , chemistry , benzene , enzyme , nad+ kinase , tetramer , glycerol , dehydrogenase , stereochemistry , molecular mass , biochemistry , organic chemistry
A second and novel cis ‐benzene dihydrodiol dehydrogenase which is able to dehydrogenate a range of cis ‐dihydrodiols and other vicinal alcohols has been purified from Pseudomonas putida ML2. The enzyme is a tetramer of a polypeptide of 39 kDa in molecular mass and has a pH optimum of 9.0. Despite having a primary structure that has significant similarity to glycerol dehydrogenases, the k cat / K m value of the enzyme for cis ‐benzene dihydrodiol is 4300‐fold higher compared to glycerol. The apparent K m values of the enzyme for cis ‐benzene dihydrodiol and glycerol are 0.01 mM and 46 mM, respectively, and 0.22 mM for NAD + .