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The vacuolar Ca 2+ /H + exchanger Vcx1p/Hum1p tightly controls cytosolic Ca 2+ levels in S. cerevisiae
Author(s) -
Miseta Attila,
Kellermayer Richard,
Aiello David P,
Fu Lianwu,
Bedwell David M
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00519-0
Subject(s) - vacuole , cytosol , extracellular , saccharomyces cerevisiae , transporter , biophysics , biochemistry , chemistry , atpase , yeast , calcium , microbiology and biotechnology , biology , cytoplasm , enzyme , gene , organic chemistry
It is well established that the vacuole plays an important role in the cellular adaptation to growth in the presence of elevated extracellular Ca 2+ concentrations in Saccharomyces cerevisiae . The Ca 2+ ATPase Pmc1p and the Ca 2+ /H + exchanger Vcx1p/Hum1p have been shown to facilitate Ca 2+ sequestration into the vacuole. However, the distinct physiological roles of these two vacuolar Ca 2+ transporters remain uncertain. Here we show that Vcx1p can rapidly sequester a sudden pulse of cytosolic Ca 2+ into the vacuole, while Pmc1p carries out this function much less efficiently. This finding is consistent with the postulated role of Vcx1p as a high capacity, low affinity Ca 2+ transporter and suggests that Vcx1p may act to attenuate the propagation of Ca 2+ signals in this organism.