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Defective binding of IRFs to the initiator element of interleukin‐1β‐converting enzyme (ICE) promoter in an interferon‐resistant Daudi subline
Author(s) -
Iwase Satsuki,
Furukawa Yusuke,
Kikuchi Jiro,
Saito Shinobu,
Nakamura Mitsuru,
Nakayama Ritsuko,
Horiguchi-Yamada Junko,
Yamada Hisashi
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00515-3
Subject(s) - microbiology and biotechnology , transcription (linguistics) , gene , chemistry , promoter , interferon gamma , interferon , messenger rna , alpha interferon , biology , gene expression , cytokine , immunology , biochemistry , philosophy , linguistics
To investigate mechanisms of interferon (IFN) resistance, we have established an IFN‐resistant Daudi subline (Daudi res ), which is 1×10 4 times more resistant to IFN‐α than parental cells. Among the IFN‐inducible genes examined, only ICE mRNA expression was deficient in Daudi res cells. We then analyzed the regulatory mechanisms of ICE transcription, and found that IFN‐induced activation of the ICE promoter was dependent on the binding of IRFs to its initiator (Inr) element. Inr binding of IRFs was markedly diminished in Daudi res cells, and forced expression of IRF‐1 was able to activate the ICE promoter to the level of parental cells. These results suggest that IRFs and their target genes, as represented by ICE in this study, are involved in IFN resistance.