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Selective peroxisome degradation in Yarrowia lipolytica after a shift of cells from acetate/oleate/ethylamine into glucose/ammonium sulfate‐containing media
Author(s) -
Gunkel Katja,
van der Klei Ida J.,
Barth Gerold,
Veenhuis Marten
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00513-x
Subject(s) - yarrowia , peroxisome , ethylamine , biochemistry , yeast , chemistry , amine oxidase , vacuole , degradation (telecommunications) , biology , enzyme , organic chemistry , cytoplasm , gene , telecommunications , computer science
We have shown that peroxisomes of the yeast Yarrowia lipolytica are subject to specific degradation after exposure of acetate/oleate‐grown cells to glucose excess conditions. Electron microscopic analysis has revealed that the peroxisomes were degraded by uptake in the vacuole. Our results suggest that peroxisomes are taken up by macroautophagic processes, because sequestration of individual peroxisomes, which occurs typically at the beginning of microautophagy, was never observed. The observation that a peroxisomal amine oxidase activity is specifically induced by ethylamine was used for the development of a plate assay screening procedure to isolate peroxisome degradation‐defective mutants.