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Induction of oxidative DNA damage in anaerobes
Author(s) -
Takeuchi Toru,
Nakaya Yasuhiro,
Kato Naoki,
Watanabe Kunitomo,
Morimoto Kanehisa
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00497-4
Subject(s) - catalase , bacteroides fragilis , superoxide dismutase , microbiology and biotechnology , oxidative stress , chemistry , dna damage , anaerobic exercise , salmonella , biology , dna , biochemistry , bacteria , antibiotics , genetics , physiology
We compared oxidative DNA damage in strictly anaerobic Prevotella melaninogenica , aerotolerant anaerobic Bacteroides fragilis , and facultative anaerobic Salmonella typhimurium after exposure to O 2 or H 2 O 2 . Using HPLC with electrochemical detection, we measured 8‐hydroxydeoxyguanosine (8OHdG) as a damage marker. O 2 induced 8OHdG in P. melaninogenica but not in B. fragilis , which shows catalase activity, or in S. typhimurium . In P. melaninogenica , with catalase, O 2 induced less 8OHdG; superoxide dismutase had no effect; with glucose and glucose oxidase, O 2 induced more 8OHdG. H 2 O 2 also markedly increased 8OHdG. O 2 was suggested to induce 8OHdG through H 2 O 2 . O 2 or H 2 O 2 decreased survival only in P. melaninogenica . Highly sensitive to oxidative stress, P. melaninogenica could prove useful for investigating oxidative DNA damage.