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Identification and cloning of TWIK‐originated similarity sequence (TOSS): a novel human 2‐pore K + channel principal subunit
Author(s) -
Pountney David J.,
Gulkarov Iosif,
Vega-Saenz de Miera Eleazar,
Holmes Douglas,
Saganich Michael,
Rudy Bernardo,
Artman Michael,
Coetzee William A.
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00495-0
Subject(s) - cloning (programming) , protein subunit , identification (biology) , computational biology , sequence (biology) , similarity (geometry) , channel (broadcasting) , microbiology and biotechnology , chemistry , biology , genetics , computer science , artificial intelligence , gene , computer network , botany , image (mathematics) , programming language
We have identified and cloned a new member of the mammalian tandem pore domain K + channel subunit family, TWIK‐originated similarity sequence, from a human testis cDNA library. The 939 bp open reading frame encodes a 313 amino acid polypeptide with a calculated Mr of 33.7 kDa. Despite the same predicted topology, there is a relatively low sequence homology between TWIK‐originated similarity sequence and other members of the mammalian tandem pore domain K + channel subunit family group. TWIK‐originated similarity sequence shares a low (<30%) identity with the other mammalian tandem pore domain K + channel subunit family group members and the highest identity (34%) with TWIK‐1 at the amino acid level. Similar low levels of sequence homology exist between all members of the mammalian tandem pore domain K + channel subunit family. Potential glycosylation and consensus PKC sites are present. Northern analysis revealed species and tissue‐specific expression patterns. Expression of TWIK‐originated similarity sequence is restricted to human pancreas, placenta and heart, while in the mouse, TWIK‐originated similarity sequence is expressed in the liver. No functional currents were observed in Xenopus laevis oocytes or HEK293T cells, suggesting that TWIK‐originated similarity sequence may be targeted to locations other than the plasma membrane or that TWIK‐originated similarity sequence may represent a novel regulatory mammalian tandem pore domain K + channel subunit family subunit.