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Involvement of cis ‐acting repressive element(s) in the 3′‐untranslated region of human connective tissue growth factor gene
Author(s) -
Kubota Satoshi,
Hattori Takako,
Nakanishi Tohru,
Takigawa Masaharu
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00480-9
Subject(s) - gene , untranslated region , three prime untranslated region , connective tissue , biology , genetics , microbiology and biotechnology , messenger rna
To analyze the regulatory mechanism of connective tissue growth factor expression, the 3′‐untranslated region (3′‐UTR) of CTGF cDNA was amplified from HeLa cell RNA. Direct nucleotide sequencing revealed a single major population in the amplicon, which was nearly identical to other sequences. Subsequently, the effect of the 3′‐UTR on gene expression was evaluated. When it was fused downstream of a firefly luciferase gene, the 3′‐UTR strongly repressed luciferase gene expression. Interestingly, the repressive effect of the antisense 3′‐UTR appeared to be more prominent than that of the sense one. Together with the fact that several consensus sequences for regulatory elements are found in it, these results suggest the involvement of multiple sets of regulatory elements in the CTGF 3′‐UTR.