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Spontaneous rearrangements in RNA sequences
Author(s) -
Chetverina Helena V,
Demidenko Alexander A,
Ugarov Victor I,
Chetverin Alexander B
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00469-x
Subject(s) - rna , rna dependent rna polymerase , bacteriophage , sequence (biology) , biology , chemistry , genetics , stereochemistry , microbiology and biotechnology , gene , escherichia coli
The ability of RNAs to spontaneously rearrange their sequences under physiological conditions is demonstrated using the molecular colony technique, which allows single RNA molecules to be detected provided that they are amplifiable by the replicase of bacteriophage Qβ. The rearrangements are Mg 2+ ‐dependent, sequence‐non‐specific, and occur both in trans and in cis at a rate of 10 −9 h −1 per site. The results suggest that the mechanism of spontaneous RNA rearrangements differs from the transesterification reactions earlier observed in the presence of Qβ replicase, and have a number of biologically important implications.