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Rpn4p acts as a transcription factor by binding to PACE, a nonamer box found upstream of 26S proteasomal and other genes in yeast
Author(s) -
Mannhaupt Gertrud,
Schnall Ralf,
Karpov Vadim,
Vetter Irene,
Feldmann Horst
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00467-6
Subject(s) - promoter , proteasome , transcription factor , reporter gene , gene , yeast , biology , electrophoretic mobility shift assay , upstream activating sequence , response element , sequence motif , transcription (linguistics) , microbiology and biotechnology , genetics , gene expression , linguistics , philosophy
We identified a new, unique upstream activating sequence (5′‐GGTGGCAAA‐3′) in the promoters of 26 out of the 32 proteasomal yeast genes characterized to date, which we propose to call proteasome‐associated control element. By using the one‐hybrid method, we show that the factor binding to the proteasome‐associated control element is Rpn4p, a protein containing a C2H2‐type finger motif and two acidic domains. Electrophoretic mobility shift assays using proteasome‐associated control element sequences from two regulatory proteasomal genes confirmed specific binding of purified Rpn4p to these sequences. The role of Rpn4p to function as a transregulator in yeast is corroborated by its ability of stimulating proteasome‐associated control element‐driven lacZ expression and by experiments using the RPT4 and RPT6 gene promoters coupled to the bacterial cat gene as a reporter. Additionally, we found the proteasome‐associated control element to occur in a number of promoters to genes which are related to the ubiquitin‐proteasome pathway in yeast.